We are happy to announce the release of SPHIRE 1.4!

This release adds GUI support for GPU ISAC and makes SPHIRE fully compatible with TranSPHIRE, allowing you to create on-the-fly reconstructions during data acquisition.

As usual, this release is a combined release of SPHIRE, EMAN2, and SPARX.

The key additions to the SPHIRE package in this update include:

• SPHIRE: The GUI now allows easy access to the SPHIRE signal subtraction pipeline to be used after 3D refinement.
• SPHIRE: Easy access to mFSC (mask-independent, local resolution capable FSC) via the SPHIRE GUI (mFSC developed by Pawel Penczek).
• SPHIRE: GPU ISAC can now be linked with and used from the SPHIRE GUI.

• AutoSPHIRE: Further improved processing of helical data in Helical Mode.
• AutoSPHIRE: Fully compatible and ready to be used with TranSPHIRE to on-the-fly create initial reconstructions during data acquisition.

• SPHIRE internal: SPHIRE has been ported to Python 3.
• SPHIRE internal: A wide range of internal changes, including:
  • multiple optimizations for improved runtime,
  • multiple bug fixes for increased stability,
  • multiple improvements for better results.

• Find the SPHIRE documentation here.
• Find the SPHIRE tutorial (direct link to .pdf file) here. (The SPHIRE tutorial is a step-by-step walkthrough of processing a data set from beginning to end, including pre-processed data milestones.)

The SPHIRE installer uses Anaconda, which installs independently of your choice of Linux distribution.

• Linux installer
• Mac installer (expermential)

Find our additional SPHIRE tools here:

• crYOLO for picking (download).
• Cinderella for 2D class selection (download).
• GPU ISAC for 2D clustering (download).
• Unblur ships with (cisTEM).

Find the installation instructions here:

• SPHIRE binary installation instructions
• SPHIRE source code installation instructions

Older and newer versions:

• Installation instructions versions