If you want to dig deep into the SPHIRE pipeline then you are in the right place. This Wiki will provide you with information about the major programs within SPHIRE and how to use them. Of course, you will also find information about installation, additional utilities, frequently asked questions and known issues. In addition, you can find usage tutorials and SPHIRE's version history. Just follow the links in the sidebar. Happy cryo-EM'ing!
Short application info
Movie frame alignment with Unblur and drift evaluation tools.
CTF estimation and graphical evaluation tool.
Particle picking and extraction.
Reproducible class average calculation.
Reproducible ab initio model calculation.
Automatic high-resolution 3D refinement.
Reproducible 3D clustering.
Real-space local resolution calculation.
Tools and scripts to make your life easier.
Main project settings, such as name, pixel, and particles sizes.