From the fairy tale Cinderella

Our binary classification tool (Cinderella) is based on a deep learning network to classify class averages, micrographs or subtomograms into good and bad categories. For class averages, it supports .hdf/.mrcs, for micrographs .mrc format and for subtomograms it expect that they are saved in a .hdf file. Cinderella was written to automate cryo-em data processing. It's open source and easy to use. We provide a pretrained general model for classifying class averages.(see tutorial). But you can easily train it with your own set of classes/micrographs/subtomograms.

Our model was trained on a set of 2D classes from ISAC. During the creation of the training dataset, I tried to ask myself “Which class would I select If I would not know the particle?” to decide which is a good class. Here are a couple of examples for good / bad classes in Cinderella:

You can easily contribute your own classes!

Right now our model is trained on 20 datasets. But we will increase the number often!

Version: 0.6.0

Uploaded: 10. March 2020

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Uploaded: 10. March 2020, Datasets: 22

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Old versions of cinderella and the pretrained model can be found in the archive

• Fix an issue for classes in mrcs format
• Minor changes

• Add support for subtomograms
• Faster file reading

• Balances unbalanced training datasets.
• It is now possible to train Cinderella to select micrographs
• Updated the general model for 2D class selection.

• Downgrade to tensorflow 1.10.1 again, as user report long initialization times
• Only report the number of good / bad classes + their fraction.

• More data augmentation (add rotation)
• Better sampling of validation data. It is now ensured that each file contributes some validation data.
• Updated tensorflow to 1.12.3 to make it compatible to the crYOLO environment

Here is the repository of our training data:

Download the public training data

Unfortunately, we cannot upload the complete training dataset, as some classes are from projects that are not yet published.

If you want to contribute with your own classes, please upload them here:

Contribute good / bad classes

Ideally, please upload separate HDF/mrcs files for good and bad classes. You can do this separation with EMAN2's e2display. However, you can also upload the classes without separation and we will try to do it.

The following instructions assume that pip and anaconda or miniconda are available. In case you have a old cinderella environment installed, first remove the old one with:

conda env remove --name cinderella

After that, create a new virtual environment:

conda create -n cinderella -c anaconda python=3.6 pyqt=5 cudnn=7.1.2 numpy==1.14.5

Activate the environment:

source activate cinderella

Install Cinderella for GPU:

pip install cinderella[gpu]

… or CPU:

pip install cinderella[cpu]

We created three tutorials:

• How to use Cinderella for 2D class selection
• How to use Cinderella for micrograph selection
• How to use Cinderella for subtomogram selection