SPHIRE 1.4 / EMAN 2.9

We are happy to announce the release of SPHIRE 1.4!

This release adds GUI support for GPU ISAC and makes SPHIRE fully compatible with TranSPHIRE, allowing you to create on-the-fly reconstructions during data acquisition.

As usual, this release is a combined release of SPHIRE, EMAN2, and SPARX.

What's new

The key additions to the SPHIRE package in this update include:

SPHIRE: The GUI now allows easy access to the SPHIRE signal subtraction pipeline to be used after 3D refinement.
SPHIRE: Easy access to mFSC (mask-independent, local resolution capable FSC) via the SPHIRE GUI (mFSC developed by Pawel Penczek).
SPHIRE: GPU ISAC can now be linked with and used from the SPHIRE GUI.

AutoSPHIRE: Further improved processing of helical data in Helical Mode.
AutoSPHIRE: Fully compatible and ready to be used with TranSPHIRE to on-the-fly create initial reconstructions during data acquisition.

SPHIRE internal: SPHIRE has been ported to Python 3.
SPHIRE internal: A wide range of internal changes, including:
- multiple optimizations for improved runtime,
- multiple bug fixes for increased stability,
- multiple improvements for better results.

Find the SPHIRE documentation here.
Find the SPHIRE tutorial (direct link to .pdf file) here. (The SPHIRE tutorial is a step-by-step walkthrough of processing a data set from beginning to end, including pre-processed data milestones.)

The installer uses Anaconda, which should install independently of the choice of Linux distribution. The Mac installer is experimental.

Here are the downloads for Mac and Linux:

SPHIRE does not support Windows

These external tools will be needed to run all tools in SPHIRE:

TranSPHIRE for automatated preprocessing

Furthermore, we recommend to use TranSPHIRE for automated preprocessing: TranSPHIRE GitHub page

Find the installation instructions here:

Older and newer versions: