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pipeline:subtract:start
This version is outdated by a newer approved version.
This version (2021/02/12 11:22) was approved by shaikh.The Previously approved version (2021/02/01 17:26) is available.
This version (2021/02/12 11:22) was approved by shaikh.The Previously approved version (2021/02/01 17:26) is available.This is an old revision of the document!
Table of Contents
Signal subtraction
What do I need?
This protocol assumes that you have run Meridien.
Steps
- Average and Filter
- Average and low-pass filter a map for segmentation.
- Segment Filtered Map
- Display the map in Chimera, and locate regions to be subtracted.
- Generate and Apply Mask
- Make a soft mask of the region to be excluded.
- Project and Subtract
- Compute re-projections of map to be subtracted, and subtract them from the original images.
- Compute 3D Reconstruction
- Compute a 3D reconstruction using refinement iteration used for signal-subtraction.
- Center Map
- Center map of remaining density.
What do I do next?
Once these steps have been completed, perform a restricted refinement.
Author / Maintainer
Tapu Shaikh
Based on protocol developed by Zhong Huang and Pawel Penczek
pipeline/subtract/start.1613125344.txt.gz ยท Last modified: 2021/02/12 11:22 by shaikh
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