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If you want to dig deep into the SPHIRE pipeline then you are in the right place. This Wiki will provide you with information about the major programs within SPHIRE and how to use them. Of course, you will also find information about installation, additional utilities, frequently asked questions and known issues. In addition, you can find usage tutorials and SPHIRE's version history. Just follow the links in the sidebar.
Happy cryo-EM'ing!
Projects Main project settings, such as name, pixel, and particles sizes. | |
Cter CTF estimation and graphical evaluation tool. | |
Window Particle picking and extraction. | |
ISAC Reproducible class average calculation. | |
Viper Reproducible ab initio model calculation. | |
Meridien Automatic high-resolution 3D refinement. | |
Sort3D Reproducible 3D clustering. | |
Subtract Signal subtraction. | |
LocalRes Real-space local resolution calculation. | |
Movie Movie frame alignment with Unblur and drift evaluation tools. | |
Utilities Tools and scripts to make your life easier. |