If you want to dig deep into the SPHIRE pipeline then you are in the right place. This Wiki will provide you with information about the major programs within SPHIRE and how to use them. Of course, you will also find information about installation, additional utilities, frequently asked questions and known issues. In addition, you can find usage tutorials and SPHIRE's version history. Just follow the links in the sidebar. Happy cryo-EM'ing!

Project

Main project settings, such as name, pixel, and particles sizes.

Cter

CTF estimation and graphical evaluation tool.

Window

Particle picking and extraction.

Isac

Reproducible class average calculation.

Viper

Reproducible ab initio model calculation.

Meridien

Automatic high-resolution 3D refinement.

Sort3D

Reproducible 3D clustering.

LocalRes

Real-space local resolution calculation.

Movie (Optional)

Movie frame alignment with Unblur and drift evaluation tools.

Utilities

Tools and scripts to make your life easier.