We are happy to announce the release of SPHIRE 1.3.

Helical processing with SPHIRE 1.3

Per particle CTF refinement. The image shows a refinement of appoferritin dataset with tilted stage. The refined defoucs values (left side) clearly shows the tilt axis. The right side is the a error estimate for every defocus value.

crYOLO can now directly used from SPHIRE 1.3

• We add support for processing helical specimens (Beta).

• Automatic processing with AutoSPHIRE: While the individual steps can be configured and executed by hand, SPHIRE 1.3 includes AutoSPHIRE, an integrated tool that enables the pipeline to run by itself up until the creation of a high resolution reconstruction.

• Per particle CTF refinement with error estimation. In the new SPHIRE version, after you reached a relative high resolution (~4A) you can refine your CTF particle wise. This can improve the resolution up to 0.3A.

• We integrated the crYOLO particle picking procedure. It's based on convolutional neural networks, very accurate and very fast.

• Visualization of 2D power spectra in CTER

• Minor bug fixes

• SPHIRE tutorial (Needs update)

The installer uses Anaconda, which should install independently of the choice of Linux distribution. The Mac installer is experimental.

Here are the downloads for Max and Linux:

• Linux installer (Needs update)
• Mac installer (Expermential)(Needs update)

These external tools will be needed to run all tools in SPHIRE:

• crYOLO download
• Unblur homepage

Here are the installation instructions:

• SPHIRE Installation instructions (Needs update)