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After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following:
The folder micrographs
containing your images. In this example the filenames are Actin-ADP-BeFx_NUMBER.mrc.
Furthermore I assume you have a folder picked_boxes
which contain your box files in helicon format. This is how you import them into relion:
1. Move the box files from picked_boxes
into the micrographs
directory.
2. Start Relion
3. We start with importing the box files. Select the import action. The Node type is 2D/3D particle coordinates. The input files pointing to your box files in the micrographs
directory, where the running number is replaced by a *. Press Run!
4. Now we import the corresponding mrc files. Select the Import action again. The Node type is 2D micrographs/tomogram. The input files pointing to your mrc files in the micrographs
directory, where the running number is replaced by a *. Press Run!
5. Now we extract the particles. Select the Particle extraction action. Select the micrographs.star file for 'micrograph STAR file' and select the coords_suffix.box for Input coordinates.
6. Select the Helix tab, set Extract helical segments to Yes and Coordinates are start-end only to No. Press Run!
Now you have extracted your filament particles