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This version is outdated by a newer approved version.DiffThis version (2021/02/12 11:22) was approved by shaikh.The Previously approved version (2021/02/01 17:26) is available.Diff

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Signal subtraction

What do I need?

This protocol assumes that you have run Meridien.


Average and Filter
Average and low-pass filter a map for segmentation.
Segment Filtered Map
Display the map in Chimera, and locate regions to be subtracted.
Generate and Apply Mask
Make a soft mask of the region to be excluded.
Project and Subtract
Compute re-projections of map to be subtracted, and subtract them from the original images.
Compute 3D Reconstruction
Compute a 3D reconstruction using refinement iteration used for signal-subtraction.
Center Map
Center map of remaining density.

What do I do next?

Once these steps have been completed, perform a restricted refinement.

Author / Maintainer

Tapu Shaikh

Based on protocol developed by Zhong Huang and Pawel Penczek

pipeline/subtract/start.1613125344.txt.gz ยท Last modified: 2021/02/12 11:22 by shaikh