SPHIRE 1.4 / EMAN 2.9

We are happy to announce the release of SPHIRE 1.4!

This release adds GUI support for GPU ISAC and makes SPHIRE fully compatible with TranSPHIRE, allowing you to create on-the-fly reconstructions during data acquisition.

As usual, this release is a combined release of SPHIRE, EMAN2, and SPARX.

The key additions to the SPHIRE package in this update include:

• SPHIRE: The GUI now allows easy access to the SPHIRE signal subtraction pipeline to be used after 3D refinement.
• SPHIRE: Easy access to mFSC (mask-independent, local resolution capable FSC) via the SPHIRE GUI (mFSC developed by Pawel Penczek).
• SPHIRE: GPU ISAC can now be linked with and used from the SPHIRE GUI.

• AutoSPHIRE: Further improved processing of helical data in Helical Mode.
• AutoSPHIRE: Fully compatible and ready to be used with TranSPHIRE to on-the-fly create initial reconstructions during data acquisition.

• SPHIRE internal: SPHIRE has been ported to Python 3.
• SPHIRE internal: A wide range of internal changes, including:
  • multiple optimizations for improved runtime,
  • multiple bug fixes for increased stability,
  • multiple improvements for better results.

• Find the SPHIRE documentation here.
• Find the SPHIRE tutorial (direct link to .pdf file) here. (The SPHIRE tutorial is a step-by-step walkthrough of processing a data set from beginning to end, including pre-processed data milestones.)

The SPHIRE installer uses Anaconda, which installs independently of your choice of Linux distribution.

• Linux installer
• Mac installer (experimental)

Find our additional SPHIRE tools here:

• crYOLO for picking (download).
• Cinderella for 2D class selection (download).
• GPU ISAC for 2D clustering (download).
• Unblur ships with (cisTEM).

Find the installation instructions here:

• SPHIRE binary installation instructions
• SPHIRE source code installation instructions

Older and newer versions:

• Installation instructions versions