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howto:sphire_helical
This version is outdated by a newer approved version.
This version (2019/09/29 14:59) is a draft.
Approvals: 0/1The Previously approved version (2019/09/27 17:45) is available.
This version (2019/09/29 14:59) is a draft.Approvals: 0/1The Previously approved version (2019/09/27 17:45) is available.
This is an old revision of the document!
How to use SPHIRE Helical
This is the very first HowTo for filamentous reconstruction.
Note that we do not impose or require any information about the helical symmetry of your data.
—
Another more detailed Tutorial including a filamentous test dataset will follow soon.
Before you start the processing helical data, you have to switch to the Helical version of SPHIRE.
You can do that in the general settings area.
► Please press the [Switch to helical GUI] button
You will notice that the logo in the lower left corner changes:
Fill out the information you have about your Protein:
Follow the steps of the normal SPA Tutorial for the steps up to the 3D refinement.
► Please press the [3D Refinement (Alpha)] button
howto/sphire_helical.1569761970.txt.gz · Last modified: 2019/09/29 14:59 by mstabrin
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