Another more detailed Tutorial including a filamentous test dataset will follow soon.
Before you start the processing helical data, you have to switch to the Helical version of SPHIRE.
You can do that in the general settings area or start the GUI with sphire --helical.
You will notice that the logo in the lower left corner changes:
Fill out the information you have about your Protein:
Follow the steps of the normal SPA Tutorial for the steps up to the 3D refinement. The information about the filament width should be filled out by default for these steps.
When you reached the step for 3D refinement:
Fill out the information like in the SPA tutorial.
A short description for the additional options:
A more detailed tutorial combined with a test dataset will follow soon.
If you encounter any problems or in case you have questions about the procedure feel free to ask in the SPHIRE mailing list or write me directly: email@example.com