After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following:
micrographscontains your images. In this example the filenames are Actin-ADP-BeFx_NUMBER.mrc.
picked_boxescontains your box files in helicon format.
The following illustrates how you import the micrographs and box files into Relion:
1. Move the box files from
picked_boxes into the
micrographs directory. The box files and the micrographs should be in the same directory before importing them into Relion.
2. Start Relion
3. We will start by importing the box files. Select the Import action. Under Node type, select 2D/3D particle coordinates. Under input files, point to your box files in the
micrographs directory, where everything starting from the running number is replaced by a * (except the suffix (e.g. _autopick.box), if one exists). Press Run!
4. Now we import the corresponding MRC files. Select the Import action again. Under Node type, enter 2D micrographs/tomogram. Under input files, point to your mrc files in the
micrographs directory, where the running number is replaced by a *. Press Run!
5. Now we will extract the particles. Select the Particle extraction action. For 'micrograph STAR file', select the micrographs.star file, and for Input coordinates select coords_suffix.box.
Use the micrographs.star file from your CTF estimation. It provides necessary information for further processing.
Alternatively, you can set your pixelsize manually in the I/O tab of the particle extraction job.
6. Go to the extract tab and set your Particle box size.
Now you have extracted your filament particles