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howto:sphire_helical [2019/09/29 15:13]
mstabrin
howto:sphire_helical [2019/10/01 12:42] (current)
twagner
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 {{ :howto:helical_project_filled.png?600 |}} {{ :howto:helical_project_filled.png?600 |}}
  
-<html+<note important
-<div style="background-color: #cfc ; padding: 10px; border: 1px solid green;">  +**Follow the in-depth tutorial**
-<b>&#9658; Follow the steps of the normal SPA Tutorial for the steps up to the 3D refinement. +
-The information about the filament width should be filled out by default for these steps.</b> +
-</div> +
-</html>+
  
 +Follow the steps of the normal [[downloads:sphire_1_3#tutorial|SPA Tutorial]] for the steps **up to** the 3D refinement.
 +The information about the filament width should be filled out by default for these steps.
 +</note>
 +When you reached the step for 3D refinement:
 <html> <html>
 <div style="background-color: #cfc ; padding: 10px; border: 1px solid green;">  <div style="background-color: #cfc ; padding: 10px; border: 1px solid green;"> 
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   * **Group name for chunks** - The random chunks are created based on this header entry name.   * **Group name for chunks** - The random chunks are created based on this header entry name.
   * **Outlier group ID** - Outlier calculation is performed after every iteration. For now, they are only calculated, but not applied automatically.   * **Outlier group ID** - Outlier calculation is performed after every iteration. For now, they are only calculated, but not applied automatically.
-  * **Helical rise [A]** - Approximate helical rise of the protein. This information is used to limit the x/y-shift range in every iteration during the refinement. +  * **Helical rise [A]** - Approximate helical rise of the protein. This information is used to limit the x/y-shift range in every iteration during the refinement. 
 + 
 +A more detailed tutorial combined with a test dataset will follow soon. 
 + 
 +If you encounter any problems or in case you have questions about the procedure feel free to ask in the [[howto:mailinglist|SPHIRE mailing list]] or write me directly: markus.stabrin@mpi-dortmund.mpg.de
howto/sphire_helical.1569762838.txt.gz · Last modified: 2019/09/29 15:13 by mstabrin