User Tools

Site Tools


sphire_1_4

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
Last revision Both sides next revision
sphire_1_4 [2021/02/15 14:34]
fschoenfeld
sphire_1_4 [2021/02/15 15:02]
twagner
Line 24: Line 24:
     * multiple improvements for better results.     * multiple improvements for better results.
  
-==== Documentation & Tutorial ====+===== Documentation & Tutorial =====
  
   * Find the [[http://sphire.mpg.de/wiki/doku.php|SPHIRE documentation]] here.   * Find the [[http://sphire.mpg.de/wiki/doku.php|SPHIRE documentation]] here.
   * Find the [[ftp://ftp.gwdg.de/pub/misc/sphire/sphire_1_3_tutorial/sphire_1_3.pdf|SPHIRE tutorial (direct link to .pdf file)]] here. (The SPHIRE tutorial is a step-by-step walkthrough of processing a data set from beginning to end, including pre-processed data milestones.)   * Find the [[ftp://ftp.gwdg.de/pub/misc/sphire/sphire_1_3_tutorial/sphire_1_3.pdf|SPHIRE tutorial (direct link to .pdf file)]] here. (The SPHIRE tutorial is a step-by-step walkthrough of processing a data set from beginning to end, including pre-processed data milestones.)
  
-==== Download ==== +===== Download =====
-The installer uses Anaconda, which should install independently of the choice of Linux distribution.  The Mac installer is experimental.+
  
-Here are the downloads for Mac and Linux:+The SPHIRE installer uses Anaconda, which installs independently of your choice of Linux distribution.
  
   * [[https://cryoem.bcm.edu/cryoem/static/software/release-2.9/eman2.9_sphire1.4_sparx.linux64.sh| Linux installer]]   * [[https://cryoem.bcm.edu/cryoem/static/software/release-2.9/eman2.9_sphire1.4_sparx.linux64.sh| Linux installer]]
-  * [[https://cryoem.bcm.edu/cryoem/static/software/release-2.9/eman2.9_sphire1.4_sparx.MacOS.sh| Mac installer (Expermential)]]+  * [[https://cryoem.bcm.edu/cryoem/static/software/release-2.9/eman2.9_sphire1.4_sparx.MacOS.sh| Mac installer (experimental)]] 
 + 
 +Find our additional SPHIRE tools here: 
 + 
 +  * **crYOLO** for picking ([[https://cryolo.readthedocs.io/en/latest/|download]]). 
 +  * **Cinderella** for 2D class selection ([[http://sphire.mpg.de/wiki/doku.php?id=auto_2d_class_selection|download]]). 
 +  * **GPU ISAC** for 2D clustering ([[https://sphire.mpg.de/wiki/doku.php?id=gpu_isac|download]]). 
 +  * **Unblur** ships with ([[https://cistem.org/|cisTEM]]).
  
 <note warning> <note warning>
-SPHIRE does not support Windows+SPHIRE does not support Windows.
 </note> </note>
- 
-These external tools will be needed to run all tools in SPHIRE: 
-  * [[https://cryolo.readthedocs.io/en/latest/|crYOLO download]] 
-  * [[http://sphire.mpg.de/wiki/doku.php?id=auto_2d_class_selection|Cinderella download]] 
-  * Unblur ships with [[https://cistem.org/|cisTEM]] 
-  * [[gpu_isac|GPU ISAC]] 
  
 <note tip> <note tip>
 **TranSPHIRE for automatated preprocessing** **TranSPHIRE for automatated preprocessing**
  
-Furthermore, we recommend to use TranSPHIRE for automated preprocessing: [[https://github.com/MPI-Dortmund/transphire|TranSPHIRE GitHub page]]+You can use TranSPHIRE to automate the cryo-EM pre-processing pipeline. When combined with AutoSPHIRE, you can also use TranSPHIRE to produce on-the-fly reconstructions during data acquisition ([[https://github.com/MPI-Dortmund/transphire|GitHub]], [[https://transphire.readthedocs.io/|documentation]]).
 </note> </note>
  
- +===== Installation instructions =====
-==== Installation instructions ====+
  
 Find the installation instructions here: Find the installation instructions here:
sphire_1_4.txt · Last modified: 2021/07/08 12:01 by mstabrin