pipeline:subtract:start []

pipeline:subtract:start

This version is outdated by a newer approved version.

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This version (2021/02/02 13:56) was approved by twagner.

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Signal subtraction

What do I need?

This protocol assumes that you have run Meridien.

Commands

Average and Filter: Average and low-pass filter a map for segmentation.
Segment Filtered Map: Display the map in Chimera, and locate regions to be subtracted.
Generate and Apply Mask: Make a soft mask of the region to be excluded.
Project and Subtract: Compute re-projections of map to be subtracted, and subtract them from the original images.
Compute 3D Reconstruction: Compute a 3D reconstruction using refinement iteration used for signal-subtraction.
Center Map: Center map of remaining density.

What do I do next?

Once these steps have been completed, perform a restricted refinement.

pipeline/subtract/start.1612196810.txt.gz · Last modified: 2021/02/01 17:26 by shaikh