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pipeline:subtract:start [2021/02/01 17:26] shaikh |
pipeline:subtract:start [2021/02/12 11:22] shaikh |
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===== Signal subtraction ===== | ===== Signal subtraction ===== | ||
- | === What do I need? === | + | ==== What do I need? ==== |
This protocol assumes that you have run Meridien. | This protocol assumes that you have run Meridien. | ||
- | ===== Commands ===== | + | ==== Steps ==== |
; [[avgfilt|Average and Filter]] : Average and low-pass filter a map for segmentation. | ; [[avgfilt|Average and Filter]] : Average and low-pass filter a map for segmentation. | ||
; [[segment|Segment Filtered Map]] : Display the map in Chimera, and locate regions to be subtracted. | ; [[segment|Segment Filtered Map]] : Display the map in Chimera, and locate regions to be subtracted. | ||
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; [[centershift|Center Map]] : Center map of remaining density. | ; [[centershift|Center Map]] : Center map of remaining density. | ||
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- | === What do I do next? === | + | ==== What do I do next? ==== |
Once these steps have been completed, perform a restricted refinement. | Once these steps have been completed, perform a restricted refinement. | ||
+ | |||
+ | \\ | ||
+ | ==== Author / Maintainer ==== | ||
+ | Tapu Shaikh | ||
+ | |||
+ | Based on protocol developed by Zhong Huang and Pawel Penczek | ||