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pipeline:subtract:start [2021/02/01 16:19]
shaikh
pipeline:subtract:start [2021/02/12 11:29]
shaikh
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 ===== Signal subtraction ===== ===== Signal subtraction =====
  
-=== Commands ===+==== What do I need? ==== 
 +This protocol assumes that you have run Meridien. 
 + 
 +==== Steps ====
   ; [[avgfilt|Average and Filter]] : Average and low-pass filter a map for segmentation.   ; [[avgfilt|Average and Filter]] : Average and low-pass filter a map for segmentation.
   ; [[segment|Segment Filtered Map]] : Display the map in Chimera, and locate regions to be subtracted.   ; [[segment|Segment Filtered Map]] : Display the map in Chimera, and locate regions to be subtracted.
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   ; [[centershift|Center Map]] : Center map of remaining density.   ; [[centershift|Center Map]] : Center map of remaining density.
          
 +==== What do I do next? ====
 +Once these steps have been completed, perform a restricted refinement.
 +
 +\\
 +==== Author / Maintainer ====
 +Tapu Shaikh
 +
 +Based on protocol developed by Zhong Huang and Pawel Penczek
 +
 +==== Reference ====
 +Bai XC, Yan C, Yang G, Lu P, Ma D, Sun L, Zhou R, Scheres SHW, Shi Y. (2015) "An atomic structure of human γ-secretase." //Nature// **525**: 212-217. doi: 10.1038/nature14892.
  
pipeline/subtract/start.txt · Last modified: 2021/02/12 11:29 by shaikh