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--- pipeline:subtract:start [2021/02/01 15:13]
shaikh created
+++ pipeline:subtract:start [2021/02/12 11:29]
shaikh
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 ===== Signal subtraction =====
-=== Commands ===
+==== What do I need? ====
+This protocol assumes that you have run Meridien.
+==== Steps ====
   ; [[avgfilt|Average and Filter]] : Average and low-pass filter a map for segmentation.
+  ; [[segment|Segment Filtered Map]] : Display the map in Chimera, and locate regions to be subtracted.
   ; [[sp_mask|Generate and Apply Mask]] : Make a soft mask of the region to be excluded.
   ; [[projsubtract|Project and Subtract]] : Compute re-projections of map to be subtracted, and subtract them from the original images.
@@ Line 8: / Line 12: @@
   ; [[centershift|Center Map]] : Center map of remaining density.
+==== What do I do next? ====
+Once these steps have been completed, perform a restricted refinement.
+\\
+==== Author / Maintainer ====
+Tapu Shaikh
+Based on protocol developed by Zhong Huang and Pawel Penczek
+==== Reference ====
+Bai XC, Yan C, Yang G, Lu P, Ma D, Sun L, Zhou R, Scheres SHW, Shi Y. (2015) "An atomic structure of human γ-secretase." //Nature// **525**: 212-217. doi: 10.1038/nature14892.