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pipeline:subtract:projsubtract [2021/02/01 16:08]
shaikh 		pipeline:subtract:projsubtract [2021/10/11 17:17]
shaikh
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 Note: If the "--voldir" flag is not specified, the resulting reconstructions will be written to the Meridien directory, which may overwrite existing files. Note: If the "--voldir" flag is not specified, the resulting reconstructions will be written to the Meridien directory, which may overwrite existing files.
 +
 +[{{ :pipeline:subtract:testmontage.png?600 |Example when using the optional flag "--nmontage", and low-pass filtered. At the left is a raw image. In the middle is a projection. At the right is the difference image. If there is a high-contrast feature in the difference image, something went wrong: probably a map with an incorrect intensity range was used.}}]
  
 \\ __6. [[centershift|Center map of the remaining density]] __ \\ __6. [[centershift|Center map of the remaining density]] __
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 ===== Input ===== ===== Input =====
 === Main Parameters === === Main Parameters ===
-  ; %%--%%origparts : Original particle stack before signal subtraction. (default None) +  ; %%--%%origparts : Original particle stack before signal subtraction. (default required string) 
-  ; %%--%%map2subtract : Map whose projections will be subtracted from the original images. (default None) +  ; %%--%%map2subtract : Map whose projections will be subtracted from the original images. (default required string) 
-  ; %%--%%projparams : Angles and shifts, from Meridien. (default None)+  ; %%--%%projparams : Angles and shifts, from Meridien. (default required string)
   ; %%--%%outdir : Directory where outputs will be written. (default required string)   ; %%--%%outdir : Directory where outputs will be written. (default required string)
      
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 \\ \\
 ==== Reference ==== ==== Reference ====
 +Bai XC, Yan C, Yang G, Lu P, Ma D, Sun L, Zhou R, Scheres SHW, Shi Y. (2015) "An atomic structure of human γ-secretase." //Nature// **525**: 212-217. doi: 10.1038/nature14892.
  
 \\ \\
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 Tapu Shaikh Tapu Shaikh
  
 +Based on a protocol developed by Zhong Huang and Pawel Penczek
 \\ \\
 ==== Keywords ==== ==== Keywords ====
pipeline/subtract/projsubtract.txt · Last modified: 2021/10/11 17:17 by shaikh

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