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pipeline:subtract:projsubtract [2021/02/01 15:47] shaikh created |
pipeline:subtract:projsubtract [2021/02/12 11:24] shaikh [Author / Maintainer] |
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sp_signalsubtract comprises the steps needed to subtract masked regions from a map in order to focus on the remaining features from that map. There are six steps for this process (five within SPHIRE): | sp_signalsubtract comprises the steps needed to subtract masked regions from a map in order to focus on the remaining features from that map. There are six steps for this process (five within SPHIRE): | ||
- | \\ __1. Average and low-pass filter a map for segmentation __ | + | \\ __1. [[avgfilt|Average and low-pass filter a map for segmentation]] __ |
\\ __2. Locate regions to be subtracted __ | \\ __2. Locate regions to be subtracted __ | ||
- | \\ __3. Mask a soft mask of the region to be excluded __ | + | \\ __3. [[sp_mask|Mask a soft mask of the region to be excluded]] __ |
- | \\ __4. Compute re-projections of map to be subtracted, and subtract them from the original images __: | + | \\ __4. [[projsubtract|Compute re-projections of map to be subtracted, and subtract them from the original images]] __: |
mpirun -np 16 sp_signalsubtract.py projsubtract --origparts=particle_stack --map2subtract=map_to_subtract --projparams=projection_parameters --outdir=output_subtraction_directory --nmontage=number_of_examples --inmem --saveprojs | mpirun -np 16 sp_signalsubtract.py projsubtract --origparts=particle_stack --map2subtract=map_to_subtract --projparams=projection_parameters --outdir=output_subtraction_directory --nmontage=number_of_examples --inmem --saveprojs | ||
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By the default, the intermediate projections will be deleted after subtraction. To save them, use the " | By the default, the intermediate projections will be deleted after subtraction. To save them, use the " | ||
- | \\ __5. Compute reconstruction of density remaining after signal-subtraction __: | + | \\ __5. [[pipeline: |
sp_meridien.py bdb: | sp_meridien.py bdb: | ||
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Note: If the " | Note: If the " | ||
- | \\ __6. Center map of the remaining density __ | + | [{{ : |
+ | |||
+ | \\ __6. [[centershift|Center map of the remaining density]] __ | ||
\\ | \\ | ||
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Tapu Shaikh | Tapu Shaikh | ||
+ | Based on a protocol developed by Zhong Huang and Pawel Penczek | ||
\\ | \\ | ||
==== Keywords ==== | ==== Keywords ==== |