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--- cryolo_filament_import_relion [2020/04/15 10:09]
twagner
+++ cryolo_filament_import_relion [2020/04/16 08:45]
twagner
@@ Line 14: / Line 14: @@
 . Now we import the corresponding MRC files. Select the //Import// action again. Under //Node type//, enter //2D micrographs/tomogram//. Under //input files//, point to your mrc files in the ''micrographs'' directory, where the running number is replaced by a *. Press //Run!// {{ ::02.png?500 |}}
-. Now we will extract the particles. Select the //Particle extraction// action. For 'micrograph STAR file', select the //micrographs.star// file (//Note: Use the micrograph.star file from your CTF estimation. It provides necessary information for further processing.//), and for //Input coordinates// select //coords_suffix.box//. {{ ::03.png?500 |}}
+. Now we will extract the particles. Select the //Particle extraction// action. For 'micrograph STAR file', select the //micrographs.star// file, and for //Input coordinates// select //coords_suffix.box//. <note important>
+**micrographs.star**
+ Use the micrographs.star file from your CTF estimation. It provides necessary information for further processing.
+Alternatively, you can set your pixelsize manually in the I/O tab of the particle extraction job.
+</note> {{ ::03.png?500 |}}
 . Go to the extract tab and set your Particle box size.