Here we will put the sphire tutorial that is right now in available as pdf ====== General ====== ====== 1. Software Installation ====== ===== 1.1 Install sphire and MPI support ===== ===== 1.2 Install crYOLO ===== ===== 1.3 Installation of accociated EM Software Packages ===== ====== 2. Project ====== ====== 3. Movie ====== ===== 3.1 Micrograph Movie Alignement ===== ===== 3.2 Drift Asssessment ===== ====== 4. CTER ====== ===== 4.1 CTF Estimation ===== ===== 4.2 CTF Assessment ===== ====== 5. Window ====== ===== 5.1 Particle Coordinates ===== ===== 5.2 Particle Extraction ===== ===== 5.3 Particle Stack ===== ====== 6. ISAC ====== ===== 6.1 2D Clustering ===== ===== 6.2 Beautifier ===== ===== 6.3 ISAC2 Stack Subset ===== ====== 7. VIPER ====== ===== 7.1 Initial 3D Model - RVIPER ===== ===== 7.2 Compare re-projections ===== ===== 7.3 Volume Adjustment ===== ===== 7.4 Adaptive 3d Mask ===== ====== 8. MERIDIEN ====== ===== 8.1 3D Refinement ===== ===== 8.2 PostRefiner ===== ====== 9. SORT3D ====== ===== 9.1 3D Variability ===== ===== 9.2 3D Clustering ===== ===== 9.3 Local Subset Refinement and Final Reconstruction ===== ====== 10. Advanced Refinement ====== ===== 10.1 CTF Refinement ===== ====== 11. LOCALRES ====== ===== 11.1 Local Resolution ===== ===== 11.2 Local Filtering =====