~~NOTOC~~ ===== sp_tilt_import ===== Tilt Import : Imports per-particle defocus estimates from gCTF. \\ ===== Usage ===== Usage in command line: sp_tilt_import.py input_star_files output_directory --box_size=box_size --apix=pixel_size --verbosity=verbosity \\ ===== Typical usage ===== The purpose of sp_tilt_import.py is to: : merge STAR files created by gCTF : convert coordinate files to .box format using sp_relion2sphire.py : extract particles using sp_window.py : generate SPHIRE particle stack using sp_relion2sphire.py \\ __Standard usage__: sp_tilt_import.py input_star_files output_directory --box_size=box_size \\ __Additional parameters__: sp_tilt_import.py input_star_files output_directory --box_size=box_size --apix=pixel_size --verbosity=verbosity Pixel size is purely cosmetic, for screen output. Verbosity affects the amount of text written the the screen/log file. \\ ===== Input ===== === Main Parameters === ; input_star_files : Set of STAR files written by gCTF, one for each micrograph. (default required string) ; output_directory : Directory where outputs will be written. (default required string) ; %%--%%box_size : Particle box size for extraction, in pixels. (default None) \\ === Advanced Parameters === ; %%--%%apix : Angstroms per pixel, purely cosmetic, for screen output. (default 1.0) ; %%--%%verbosity : Controls how much information is written to screen (default 1) \\ ===== Output ===== ; init_local.star : Merged STAR file from initial conversion from gCTF ; sphire_micrographs.txt : List of micrographs ; sphire_cter_partres.txt : CTF parameters for each micrograph, in CTER partres format ; Coordinates/*.box : Coordinates in box format, one for each micrograph ; Rebox/*.rbx : Rebox files, one for each micrograph ; Particles/*.mrcs : Particle stacks, one for each micrographs ; bdb:sphire_stack : SPHIRE combined particle BDB stack ; all_local.star : Merged STAR file following extraction ; ctf_params.txt : CTF parameters for each particle \\ ===== Description ===== \\ ==== Method ==== \\ ==== Reference ==== \\ ==== Developer Notes ==== \\ ==== Author / Maintainer ==== Tapu Shaikh \\ ==== Keywords ==== Category 1:: APPLICATIONS \\ ==== Files ==== \\ ==== See also ==== \\ ==== Maturity ==== Beta:: Under evaluation and testing. Please let us know if there are any bugs. \\ ==== Bugs ==== There are no known bugs so far. \\