~~NOTOC~~ ===== sxisac_post_processing ===== Beautifier: Beautify the ISAC 2D clustering result with the original pixel size. \\ ===== Usage ===== Usage in command lines: sxisac_post_processing.py stack_file isac_directory class_file_name_no_dir_info --radius=particle_radius --CTF --single_stack_output \\ ===== Typical usage ===== sxisac_post_processing.py exists only in MPI version. mpirun -np 32 sxisac_post_processing.py bdb:data fisac1 locres.hdf --radius=28 \\ ===== Input ===== === Main Parameters === ; stack_file : Original image stack: Particles required to create the full-sized class averages. The images must be square (''nx''=''ny'') and the stack must be bdb format (default required string) ; isac_directory : Isac output directory: Name of the directory where isac was run previously. (default required string) ; class_file_name_no_dir_info : Isac class file name: Name of the file (no directory info) that contains the class averages. It is located in the Isac directory. (default required string) ; radius : Particle radius [Pixels]: There is no default radius. (default required int) ; CTF : Phase-flip: If set, the data will be phase-flipped using CTF information included in the image headers. (default False) ; single_stack_output : Single stack output: If set, only one stack for all classes will be generated. Otherwise, which is the default, one file per class will be generated. (default False) \\ ===== Output ===== The output directory is created in the isac directory and has the format "post_processing_YYYY-MM-DD--HH-MM-SS". It contains all the output files "original_image_number_in_each_class_average.txt", "twoD_params_info_list.txt" and "radius_adjusted_class_averages.hdf" (one file or multiple files according to the --single_stack_output option). \\ ==== Author / Maintainer ==== Horatiu Voicu and Pawel A. Penczek \\ ==== Files ===== sxisac_post_processing.py \\ ==== Maturity ==== alpha:: in development \\