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howto:sphire_helical [2019/09/27 17:19]
twagner
howto:sphire_helical [2019/09/29 14:59]
mstabrin
Line 1: Line 1:
 ====== How to use SPHIRE Helical ====== ====== How to use SPHIRE Helical ======
  
-Before you start the processing helical data, you have to switch to the Helical version of SPHIRYour do that in the general settings area.+<note> 
 +This is the very first HowTo for filamentous reconstruction.   
 +Note that we do not impose or require any information about the helical symmetry of your data.   
 + 
 +--- 
 + 
 +Another more detailed Tutorial including a filamentous test dataset will follow soon. 
 +</note> 
 + 
 +Before you start the processing helical data, you have to switch to the Helical version of SPHIRE 
 + 
 +You can do that in the general settings area. 
 + 
 +<html> 
 +<div style="background-color: #cfc ; padding: 10px; border: 1px solid green;">  
 +<b>&#9658; Please press the [Switch to helical GUI] button</b> 
 +</div> 
 +</html> 
 {{ :howto:sphire_normal.png?600 |}} {{ :howto:sphire_normal.png?600 |}}
  
-{{ :howto:sphire_helical.png?600 |}}+You will notice that the logo in the lower left corner changes: 
 + 
 +{{ :howto:sphire_helical_arrow.png?600 |}} 
 + 
 +Fill out the information you have about your Protein: 
 + 
 +{{ :howto:helical_project_filled.png?600 |}} 
 + 
 +Follow the steps of the normal SPA Tutorial for the steps up to the 3D refinement. 
 + 
 +<html> 
 +<div style="background-color: #cfc ; padding: 10px; border: 1px solid green;">  
 +<b>&#9658; Please press the [3D Refinement (Alpha)] button</b> 
 +</div> 
 +</html>
  
 +{{ :howto:sphire_helical_meridien.png?600 |}}
howto/sphire_helical.txt · Last modified: 2019/10/01 12:42 by twagner