This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
cryolo_filament_import_relion [2018/12/18 13:34] shaikh |
cryolo_filament_import_relion [2020/04/15 10:06] twagner |
||
---|---|---|---|
Line 1: | Line 1: | ||
====== How to import crYOLO filament coordinates into Relion ====== | ====== How to import crYOLO filament coordinates into Relion ====== | ||
After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following: | After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following: | ||
- | * The folder '' | + | * The folder '' |
- | * Furthermore I assume you have a folder '' | + | * The folder '' |
The following illustrates how you import the micrographs and box files into Relion: | The following illustrates how you import the micrographs and box files into Relion: | ||
Line 10: | Line 10: | ||
2. Start Relion | 2. Start Relion | ||
- | 3. We will start with importing the box files. Select the //Import// action. Under //Node type//, select //2D/3D particle coordinates// | + | 3. We will start by importing the box files. Select the //Import// action. Under //Node type//, select //2D/3D particle coordinates// |
4. Now we import the corresponding MRC files. Select the //Import// action again. Under //Node type//, enter //2D micrographs/ | 4. Now we import the corresponding MRC files. Select the //Import// action again. Under //Node type//, enter //2D micrographs/ | ||
- | 5. Now we will extract the particles. Select the //Particle extraction// | + | 5. Now we will extract the particles. Select the //Particle extraction// |
- | 6. Select the //Helix// tab, set //Extract helical segments// to //Yes// and // | + | 6. Go to the extract tab and set your Particle box size. |
+ | |||
+ | 7. Select the //Helix// tab, and set //Extract helical segments// to //Yes// and // | ||
Now you have extracted your filament particles :-) | Now you have extracted your filament particles :-) |