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You can use crYOLO either by command line or by using the GUI. The GUI should be easier for most users. You can start it with:

cryolo_gui.py

The crYOLO GUI is essentially a visualization of the command line interface. On left side, you find all possible “Actions”:

• config: With this action you create the configuration file that you need to run crYOLO.
• train: This action lets you train crYOLO from scratch or refine an existing model.
• predict: If you have a (pre)trained model you can pick particles in your data set using this command.
• evaluation: This action helps you to quantify the quality of your model.

Each action has several parameters which are organized in tabs. Once you have chosen your settings you can press [Start] (just as example, don't press it now ), the command will be applied and crYOLO shows you the output:

It will tell you if something went wrong. Moreover, it will tell you all parameters used. Pressing [Back] brings you back to your settings, where you can either edit the settings (in case something went wrong) or go to the next action.