This shows you the differences between two versions of the page.
Both sides previous revision Previous revision | Next revision Both sides next revision | ||
pipeline:sort3d:sxsort3d [2018/02/23 16:59] moriya |
pipeline:sort3d:sxsort3d [2018/02/23 18:15] moriya |
||
---|---|---|---|
Line 25: | Line 25: | ||
; mask : 3D mask: File path of the global 3D mask for clustering. (default none) | ; mask : 3D mask: File path of the global 3D mask for clustering. (default none) | ||
- | ; focus : Binary Focus 3D mask: Binary 3D mask used for focused clustering. (default none) | + | ; %%--%%focus : Binary Focus 3D mask: Binary 3D mask used for focused clustering. (default none) |
- | ; radius : Particle radius [Pixels]: Used as outer radius for rotational correlation. | + | ; %%--%%radius : Particle radius [Pixels]: Used as outer radius for rotational correlation. |
- | ; delta : Angular step for projections [Degrees]: Angular step of reference projections. (default ' | + | ; %%--%%delta : Angular step for projections [Degrees]: Angular step of reference projections. (default ' |
- | ; CTF : Use CTF: Do a full CTF correction during the alignment. (default False) | + | ; %%--%%CTF : Use CTF: Do a full CTF correction during the alignment. (default False) |
- | ; sym : Point-group symmetry: Point-group symmetry of the target structure. (default c1) | + | ; %%--%%sym : Point-group symmetry: Point-group symmetry of the target structure. (default c1) |
- | ; number_of_images_per_group : Images per group: Critical value defined by user. It suggests program the number of groups. (default 1000) | + | ; %%--%%number_of_images_per_group : Images per group: Critical value defined by user. It suggests program the number of groups. (default 1000) |
- | ; nxinit : Initial image size for sorting: Initial image size for sorting. (default 64) | + | ; %%--%%nxinit : Initial image size for sorting: Initial image size for sorting. (default 64) |
- | ; smallest_group : Smallest group size: Minimum members for identified group. (default 500) | + | ; %%--%%smallest_group : Smallest group size: Minimum members for identified group. (default 500) |
- | ; chunk0 : Chunk file name for 1st halfset: Name of chunk file containing particle IDs of 1st halfset (chunk0) for computing margin of error. (default none) | + | ; %%--%%chunk0 : Chunk file name for 1st halfset: Name of chunk file containing particle IDs of 1st halfset (chunk0) for computing margin of error. (default none) |
- | ; chunk1 : Chunk file name for 2nd halfset: Name of chunk file containing particle IDs of 2nd halfset (chunk1) for computing margin of error. (default none) | + | ; %%--%%chunk1 : Chunk file name for 2nd halfset: Name of chunk file containing particle IDs of 2nd halfset (chunk1) for computing margin of error. (default none) |
\\ | \\ | ||
=== Advanced Parameters === | === Advanced Parameters === | ||
- | ; ir : Inner radius for rotational correlation [Pixels]: Must be bigger than 1. (default 1) | + | ; %%--%%ir : Inner radius for rotational correlation [Pixels]: Must be bigger than 1. (default 1) |
- | ; maxit : Maximum iterations: Maximum number of iteration. (default 25) | + | ; %%--%%maxit : Maximum iterations: Maximum number of iteration. (default 25) |
- | ; rs : Step between rings in rotational correlation: | + | ; %%--%%rs : Step between rings in rotational correlation: |
- | ; xr : X search range [Pixels]: The translational search range in the x direction will take place in -xr to +xr range. (default ' | + | ; %%--%%xr : X search range [Pixels]: The translational search range in the x direction will take place in -xr to +xr range. (default ' |
- | ; yr : Y search range [Pixels]: The translational search range in the y direction will take place in -yr to +yr range.. If omitted, it will be set as xr. (default ' | + | ; %%--%%yr : Y search range [Pixels]: The translational search range in the y direction will take place in -yr to +yr range.. If omitted, it will be set as xr. (default ' |
- | ; ts : Translational search step [Pixels]: The search will be performed in -xr, -xr+ts, 0, xr-ts, xr, can be fractional. (default ' | + | ; %%--%%ts : Translational search step [Pixels]: The search will be performed in -xr, -xr+ts, 0, xr-ts, xr, can be fractional. (default ' |
- | ; an : Local angular search width [Degrees]: This defines the neighbourhood where the local angular search will be performed. (default ' | + | ; %%--%%an : Local angular search width [Degrees]: This defines the neighbourhood where the local angular search will be performed. (default ' |
- | ; center : Centering method: 0 - if you do not want the volume to be centered, 1 - center the volume using the center of gravity. (default 0) | + | ; %%--%%center : Centering method: 0 - if you do not want the volume to be centered, 1 - center the volume using the center of gravity. (default 0) |
- | ; nassign : Number of reassignment iterations: Performed for each angular step. (default 1) | + | ; %%--%%nassign : Number of reassignment iterations: Performed for each angular step. (default 1) |
- | ; nrefine : Number of alignment iterations: Performed for each angular step. (default 0) | + | ; %%--%%nrefine : Number of alignment iterations: Performed for each angular step. (default 0) |
- | ; stoprnct : Assignment convergence threshold [%]: Used to asses convergence of the run. It is the minimum percentage of assignment change required to stop the run. (default 3.0) | + | ; %%--%%stoprnct : Assignment convergence threshold [%]: Used to asses convergence of the run. It is the minimum percentage of assignment change required to stop the run. (default 3.0) |
- | ; function : Reference preparation function: Specify name of function used to prepare the reference volume. (default do_volume_mrk05) | + | ; %%--%%function : Reference preparation function: Specify name of function used to prepare the reference volume. (default do_volume_mrk05) |
- | ; independent : Number of independent runs: Number of independent equal-Kmeans. (default 3) | + | ; %%--%%independent : Number of independent runs: Number of independent equal-Kmeans. (default 3) |
- | ; low_pass_filter : Low-pass filter frequency [1/Pixels]: Low-pass filter used for the 3D sorting on the original image size. Specify with absolute frequency. (default -1.0) | + | ; %%--%%low_pass_filter : Low-pass filter frequency [1/Pixels]: Low-pass filter used for the 3D sorting on the original image size. Specify with absolute frequency. (default -1.0) |
- | ; unaccounted : Reconstruct unaccounted images: Reconstruct unaccounted images. (default False) | + | ; %%--%%unaccounted : Reconstruct unaccounted images: Reconstruct unaccounted images. (default False) |
- | ; seed : Random seed: Seed used for the initial random assignment for EQ Kmeans. The program generates a random integer by default. (default -1) | + | ; %%--%%seed : Random seed: Seed used for the initial random assignment for EQ Kmeans. The program generates a random integer by default. (default -1) |
- | ; sausage : Use sausage filter: A way of filtering volume. (default False) | + | ; %%--%%sausage : Use sausage filter: A way of filtering volume. (default False) |
- | ; PWadjustment : Power spectrum reference: Text file containing a 1D reference power spectrum used for EM density map power spectrum correction. Typically, compute 1D power spectrum from PDB file. (default none) | + | ; %%--%%PWadjustment : Power spectrum reference: Text file containing a 1D reference power spectrum used for EM density map power spectrum correction. Typically, compute 1D power spectrum from PDB file. (default none) |
- | ; protein_shape : Protein Shape: It defines protein preferred orientation angles. " | + | ; %%--%%protein_shape : Protein Shape: It defines protein preferred orientation angles. " |
- | ; upscale : Power spectrum adjustment strength: This parameters adjusts how strongly the power spectrum of the volume should be modified to match the reference. A value of 1 brings the volume' | + | ; %%--%%upscale : Power spectrum adjustment strength: This parameters adjusts how strongly the power spectrum of the volume should be modified to match the reference. A value of 1 brings the volume' |
- | ; wn : Target image size [Pixels]: Specify optimal window size for data processing. If different than 0, then the images will be rescaled to fit this size. (default 0) | + | ; %%--%%wn : Target image size [Pixels]: Specify optimal window size for data processing. If different than 0, then the images will be rescaled to fit this size. (default 0) |
- | ; interpolation : 3D interpolation method: Method interpolation in 3D. Options are tr1 or 4nn. (default ' | + | ; %%--%%interpolation : 3D interpolation method: Method interpolation in 3D. Options are tr1 or 4nn. (default ' |
\\ | \\ | ||
Line 93: | Line 93: | ||
\\ | \\ | ||
==== See also ==== | ==== See also ==== | ||
- | [[pipeline: | + | [[pipeline: |
\\ | \\ |