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downloads:cryolo_1 [2020/02/07 13:50]
twagner [Installation]
downloads:cryolo_1 [2020/05/25 10:14]
twagner
Line 1: Line 1:
 {{ :downloads:cryolo_logo.jpg?600 |}} {{ :downloads:cryolo_logo.jpg?600 |}}
 +<note important>
 +
 +**DOCUMENTATION OUTDATED**
 +
 +The documentation has moved to https://cryolo.readthedocs.io/en/latest/
 +
 +</note>
 +
 ====== License ====== ====== License ======
  
Line 12: Line 20:
 You can find more technical details in our paper: You can find more technical details in our paper:
  
-Nature Communications Biology:+Communications Biology:
 [[https://doi.org/10.1038/s42003-019-0437-z|SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM]] [[https://doi.org/10.1038/s42003-019-0437-z|SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM]]
  
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 ====crYOLO==== ====crYOLO====
  
-Version: 1.5.6+Version: 1.6.1
  
-Uploaded:  17December 2019+Uploaded:  30March 2020
  
 [[https://pypi.org/project/cryolo/#files|DOWNLOAD]] [[https://pypi.org/project/cryolo/#files|DOWNLOAD]]
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 ====crYOLO boxmanager==== ====crYOLO boxmanager====
  
-Version: 1.2.9+Version: 1.3.5
  
-Uploaded: 09December 2019+Uploaded: 22March 2020
  
 [[https://pypi.org/project/cryoloBM/#files|DOWNLOAD]] [[https://pypi.org/project/cryoloBM/#files|DOWNLOAD]]
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 Number of datasets: 43 real, 10 simulated, 10 particle free datasets on various grids with contamination Number of datasets: 43 real, 10 simulated, 10 particle free datasets on various grids with contamination
  
-Uploaded: 15Oktober 2019+Uploaded: 16March 2020
  
-[[ftp://ftp.gwdg.de/pub/misc/sphire/crYOLO-GENERAL-MODELS/gmodel_phosnet_201912_N63.h5+[[ftp://ftp.gwdg.de/pub/misc/sphire/crYOLO-GENERAL-MODELS/gmodel_phosnet_202002_N63.h5
 |DOWNLOAD]]  |DOWNLOAD]] 
  
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 Number of datasets: 43 real, 10 simulated, 10 particle free datasets on various grids with contamination Number of datasets: 43 real, 10 simulated, 10 particle free datasets on various grids with contamination
  
-Uploaded: 15Oktober 2019+Uploaded: 17March 2020
  
-[[ftp://ftp.gwdg.de/pub/misc/sphire/crYOLO-GENERAL-MODELS/gmodel_phosnet_201912_nn_N63.h5+[[ftp://ftp.gwdg.de/pub/misc/sphire/crYOLO-GENERAL-MODELS/gmodel_phosnet_202003_nn_N63.h5
 |DOWNLOAD]]  |DOWNLOAD]] 
  
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 ====== Installation ====== ====== Installation ======
 +
 +<note important>
 +
 +**DOCUMENTATION OUTDATED**
 +
 +The documentation has moved to https://cryolo.readthedocs.io/en/latest/
 +
 +</note>
  
 **System requirements:** **System requirements:**
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 </note> </note>
  
- +{{page>pipeline:window:cryolo:issues}}
-<note important> +
-**Known issues** +
- +
-  * Issue 0Training on multiple GPUs sometimes lead to worse performance (higher loss). We currently recommend to train on single gpus. +
-  * Issue 17On the fly filtering (%%--%%otf) is slower than using it not, as the filtering is not parallelized in this case.  +
-  * Issue 27Filament mode is not working with micrographs motion corrected by unblur. Will be fixed in the next release. +
- +
- +
- +
-<hidden Closed issues+
-  * <del>  Issue 1: crYOLO sometimes not exit properly after training finished. Has to be killed manually.</del> +
-  * <del>Issue 2: If you use automatic filtering with .tif files, you get an error like "OSError: cannot identify image file 'filtered_folder/another_folder/my_image.tif'". It will be fixed in the next release.</del> +
-  * <del>Issue 3: (Boxmanager) The visualization only shows the first filament when loading eman1 helical box files (start end coordinates). Will be fixed in the next release.</del> +
-  * <del>Issue 4: The filament mode will crash if crYOLO cannot identify a single particle in the image. Will be fixed in 1.2.2</del> +
-  * <del>Issue 5: If movies were aligned with cisTEM and picked with crYOLO, the box position are vertically flipped. Will be fixed in 1.2.2</del> +
-  * <del>Issue 6: crYOLO does overwrite the environmental variable "CUDA_VISIBLE_DEVICES" with 0 if no gpu is specified by the -g parameter. This leads to the behavior that crYOLO ignores previous settings in CUDA_VISIBLE_DEVICES. Will be fixed in 1.2.2</del> +
-  * <del>Issue 7: On K3 images crYOLO seems to add a offset toward the longer axis of the input image.</del> +
-  * <del>Issue 8: There is a logical error in filament tracing, which sometimes connects two parallel filaments.</del> +
-  * <del>Issue 9: Some people report an error when running cryolo prediction/training: "ImportError: numpy.core.multiarray failed to import". It will be fixed in 1.2.3.</del> +
-  * <del>Issue 10: On machines with many cores (e.g 64) an error during filtering might pop up: "[ERROR:0] 53: Can't spawn new thread"</del> +
-  * <del>Issue 11: If the -g parameter is not provided, crYOLO will use the memory of all GPUs. Will be fixed in 1.2.3.</del> +
-  * <del>Issue 12: The LineEnhancer depdenceny of crYOLO is still dependent from opencv. Workaround: In the crYOLO environment: conda install opencv</del> +
-  * <del>Issue 13: After picking it can happen that some of the boxes are not fully immersed in the image. Will be fixed in 1.2.4.</del> +
-  * <del>Issue 14: Parallelization in filament mode is broken. Will be fixed in 1.2.4.</del> +
-  * <del>Issue 15: If the %%--%%gpu_fraction is used, crYOLO always uses GPU 0. Will be fixed in 1.3.1.</del>  +
-  * <del>Issue 16: %%--%%gpu_fraction only works for prediction, not for training. Will be fixed in 1.3.2.</del> +
-  * <del>Issue 18: Prediction is broken in 1.3.2. It removes all particles as it claim they are not fully immersed in the image.</del> +
-  * <del>Issue 19: Filtering does not work if target image directory is absolute path.</del> +
-  * <del>Issue 20: crYOLO 1.3.4 has a normalization bug. During training the images are normalized seperately, but during prediction is done batch wise. Workaround: Use -pbs 1 during prediction. It will be fixed in 1.3.5.</del> +
-  * <del>Issue 21: The search range for filament tracing is too low for many datasets. To check if you are affected: Use your trained model and pick without the filament options. Check if your filaments a nicely picked (many consecutive boxes on a filament). In the next version, the search range will be increased and added as an optional parameter.</del> +
-  * <del>Issue 22: If absolute paths are used in the field "train_image" in your configuration file, filtering is skipped.</del> +
-  * <del>Issue 23: Since crYOLO 1.4.0 it sometimes take long until it starts picking. The reason seems to be the tensorflow update.<del> +
-  * <del>Issue 24: Fine-tune mode does not start (cannot find layer model_3). Will be fixed in 1.4.1.<del> +
-  * <del>Issue 25: When using GUI, prediction behaves differently than using command line. The reason is, that it uses a different multiprocessing start method. Will be fixed with 1.5.1</del> +
-  * <del>Issue 26: If you select filtering "None" crYOLO does not train properly.</del> +
-</hidden> +
-</note> +
 **That's it!** **That's it!**
  
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 {{page>pipeline:window:cryolo:changelog}} {{page>pipeline:window:cryolo:changelog}}
 ====crYOLO Boxmanager==== ====crYOLO Boxmanager====
 +
 +**crYOLO Boxmanager 1.3.5**
 +  * Fixed a bug when placing, moving or deleting a box
 +  * Fixed bug of nun closing progress dialog when writing boxfiles
 +
 +**crYOLO Boxmanager 1.3.1**
 +  * Speed up boxfile import is now 2x faster compared to 1.3.0.
 +  * Big speed-up for live-preview during filtering. Should now even work with very big datasets.
 +
 +
 +**crYOLO Boxmanager 1.3.0**
 +  * Added option to plot size- and confidence distribution for cbox files.
 +  * Added slider to filter particles according their estimated size.
 +  * Added addition field for the number of boxes with live update.
 +  * Added wildcard commandline option.
 +  * Show progress-bar when reading and writing box-files.
 +  * Various speed-ups.
 +
 +<hidden **Old crYOLO Boxmanager change logs**>
  
 **crYOLO Boxmanager Version 1.2.9:** **crYOLO Boxmanager Version 1.2.9:**
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 **crYOLO Boxmanager Version 1.2.8:** **crYOLO Boxmanager Version 1.2.8:**
   * Add a low pass filter   * Add a low pass filter
- 
-<hidden **Old crYOLO Boxmanager change logs**> 
  
 **crYOLO Boxmanager Version 1.2.6:** **crYOLO Boxmanager Version 1.2.6:**
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 </hidden> </hidden>
 ==== General PhosaurusNet model ==== ==== General PhosaurusNet model ====
-**Version 201912:**:+ 
 +**Version 202002:** 
 +  * Adjusted the training data that the box sizes better reflect the particle diameter. 
 + 
 +**Version 201912:**
   * Added two more datasets.   * Added two more datasets.
  
-**Version 20190516:**:+**Version 20190516:**
   * Added four more inhouse datasets   * Added four more inhouse datasets
   * Added SNRNP (Thanks to Clement Charenton)    * Added SNRNP (Thanks to Clement Charenton) 
downloads/cryolo_1.txt · Last modified: 2021/02/19 09:43 by twagner