cryolo_filament_import_relion

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
cryolo_filament_import_relion [2020/04/15 10:09]
twagner
cryolo_filament_import_relion [2020/04/16 08:45] (current)
twagner
Line 14: Line 14:
 4. Now we import the corresponding MRC files. Select the //Import// action again. Under //Node type//, enter //2D micrographs/​tomogram//​. Under //input files//, point to your mrc files in the ''​micrographs''​ directory, where the running number is replaced by a *. Press //Run!// {{ ::​02.png?​500 |}}  4. Now we import the corresponding MRC files. Select the //Import// action again. Under //Node type//, enter //2D micrographs/​tomogram//​. Under //input files//, point to your mrc files in the ''​micrographs''​ directory, where the running number is replaced by a *. Press //Run!// {{ ::​02.png?​500 |}} 
  
-5. Now we will extract the particles. Select the //Particle extraction//​ action. For '​micrograph STAR file', select the //​micrographs.star//​ file (//Note: Use the micrograph.star file from your CTF estimation. It provides necessary information for further processing.//)and for //Input coordinates// select //​coords_suffix.box//. {{ ::​03.png?​500 |}}+5. Now we will extract the particles. Select the //Particle extraction//​ action. For '​micrograph STAR file', select the //​micrographs.star//​ file, and for //Input coordinates//​ select //​coords_suffix.box//​. <note important>​ 
 +**micrographs.star** 
 + 
 + Use the micrographs.star file from your CTF estimation. It provides necessary information for further processing. 
 + 
 +Alternativelyyou can set your pixelsize manually in the I/O tab of the particle extraction job. 
 +</note> ​{{ ::​03.png?​500 |}}
  
 6. Go to the extract tab and set your Particle box size. 6. Go to the extract tab and set your Particle box size.
  • cryolo_filament_import_relion.txt
  • Last modified: 2020/04/16 08:45
  • by twagner