After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following:

• The folder micrographs containing your images. In this example the filenames are Actin-ADP-BeFx_NUMBER.mrc.
• Furthermore I assume you have a folder picked_boxes which contain your box files in helicon format.

The following illustrates how you import the micrographs and box files into Relion:

1. Move the box files from picked_boxes into the micrographs directory. The box-files and the micrographs should be in the same directory before importing them into Relion.

2. Start Relion

3. We start with importing the box files. Select the import action. The Node type is 2D/3D particle coordinates. The input files pointing to your box files in the micrographs directory, where the running number is replaced by a *. Press Run!

4. Now we import the corresponding mrc files. Select the Import action again. The Node type is 2D micrographs/tomogram. The input files pointing to your mrc files in the micrographs directory, where the running number is replaced by a *. Press Run!

5. Now we extract the particles. Select the Particle extraction action. Select the micrographs.star file for 'micrograph STAR file' and select the coords_suffix.box for Input coordinates.

6. Select the Helix tab, set Extract helical segments to Yes and Coordinates are start-end only to No. Press Run!

Now you have extracted your filament particles