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cryolo_filament_import_relion [2018/12/18 11:53] shaikh |
cryolo_filament_import_relion [2020/04/16 08:45] twagner |
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====== How to import crYOLO filament coordinates into Relion ====== | ====== How to import crYOLO filament coordinates into Relion ====== | ||
After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following: | After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following: | ||
- | * The folder '' | + | * The folder '' |
- | * Furthermore I assume you have a folder '' | + | * The folder '' |
- | The following illustrates how you import | + | The following illustrates how you import |
- | 1. Move the box files from '' | + | 1. Move the box files from '' |
2. Start Relion | 2. Start Relion | ||
- | 3. We start with importing the box files. Select the import | + | 3. We will start by importing the box files. Select the // |
- | 4. Now we import the corresponding | + | 4. Now we import the corresponding |
- | 5. Now we extract the particles. Select the //Particle extraction// | + | 5. Now we will extract the particles. Select the //Particle extraction// |
+ | **micrographs.star** | ||
- | 6. Select the //Helix// tab, set //Extract helical segments// to //Yes// and // | + | Use the micrographs.star file from your CTF estimation. It provides necessary information for further processing. |
+ | |||
+ | Alternatively, | ||
+ | </ | ||
+ | |||
+ | 6. Go to the extract tab and set your Particle box size. | ||
+ | |||
+ | 7. Select the //Helix// tab, and set //Extract helical segments// to //Yes// and // | ||
Now you have extracted your filament particles :-) | Now you have extracted your filament particles :-) |