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cryolo_filament_import_relion [2018/12/18 11:53]
shaikh
cryolo_filament_import_relion [2018/12/18 11:57]
twagner
Line 4: Line 4:
   * Furthermore I assume you have a folder ''picked_boxes'' which contain your box files in helicon format.    * Furthermore I assume you have a folder ''picked_boxes'' which contain your box files in helicon format. 
  
-The following illustrates how you import them into Relion:+The following illustrates how you import the micrographs and box files into Relion:
  
 1. Move the box files from ''picked_boxes'' into the ''micrographs'' directory. The box-files and the micrographs should be in the same directory before importing them into Relion. 1. Move the box files from ''picked_boxes'' into the ''micrographs'' directory. The box-files and the micrographs should be in the same directory before importing them into Relion.
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 5. Now we extract the particles. Select the //Particle extraction// action. Select the //micrographs.star// file for 'micrograph STAR file' and select the //coords_suffix.box// for //Input coordinates//. {{ ::03.png?500 |}} 5. Now we extract the particles. Select the //Particle extraction// action. Select the //micrographs.star// file for 'micrograph STAR file' and select the //coords_suffix.box// for //Input coordinates//. {{ ::03.png?500 |}}
  
-6. Select the //Helix// tab, set //Extract helical segments// to //Yes// and //Coordinates are start-end only// to //No//. Press //Run!//{{ ::03b.png?500 |}}+6. Select the //Helix// tab, set //Extract helical segments// to //Yes// and //Coordinates are start-end only// to //No//. Press //Run!//{{ ::03_b.png?500 |}}
  
 Now you have extracted your filament particles :-) Now you have extracted your filament particles :-)
cryolo_filament_import_relion.txt ยท Last modified: 2020/04/16 08:45 by twagner