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cryolo_filament_import_relion [2018/12/17 11:30]
twagner 		cryolo_filament_import_relion [2018/12/18 11:57]
twagner
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 ====== How to import crYOLO filament coordinates into Relion ====== ====== How to import crYOLO filament coordinates into Relion ======
 After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following: After the picking of filaments with crYOLO is done, one might want to import them into Relion. In this example I assume the following:
-The folder ''micrographs'' containing your images. In this example the filenames are //Actin-ADP-BeFx_NUMBER.mrc//. +  * The folder ''micrographs'' containing your images. In this example the filenames are //Actin-ADP-BeFx_NUMBER.mrc//. 
-Furthermore I assume you have a folder ''picked_boxes'' which contain your box files in helicon format. This is how you import them into relion:+  * Furthermore I assume you have a folder ''picked_boxes'' which contain your box files in helicon format.  
 + 
 +The following illustrates how you import the micrographs and box files into Relion:
  
 1. Move the box files from ''picked_boxes'' into the ''micrographs'' directory. The box-files and the micrographs should be in the same directory before importing them into Relion. 1. Move the box files from ''picked_boxes'' into the ''micrographs'' directory. The box-files and the micrographs should be in the same directory before importing them into Relion.
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 5. Now we extract the particles. Select the //Particle extraction// action. Select the //micrographs.star// file for 'micrograph STAR file' and select the //coords_suffix.box// for //Input coordinates//. {{ ::03.png?500 |}} 5. Now we extract the particles. Select the //Particle extraction// action. Select the //micrographs.star// file for 'micrograph STAR file' and select the //coords_suffix.box// for //Input coordinates//. {{ ::03.png?500 |}}
  
-6. Select the //Helix// tab, set //Extract helical segments// to //Yes// and //Coordinates are start-end only// to //No//. Press //Run!//{{ ::03b.png?500 |}}+6. Select the //Helix// tab, set //Extract helical segments// to //Yes// and //Coordinates are start-end only// to //No//. Press //Run!//{{ ::03_b.png?500 |}}
  
 Now you have extracted your filament particles :-) Now you have extracted your filament particles :-)
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cryolo_filament_import_relion.txt · Last modified: 2020/04/16 08:45 by twagner

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