automated_helical_picking []

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.

====== Helical Picking Procedure ======

===== Overview =====
{{::screen_shot_2017-12-08_at_12.43.57.png?direct&200 |}} The helical particle procedure 

===== Installation =====
===== Get your data into ImageJ ====
=== Load the raw data ===
  - File -> Import -> Image Sequence
  - Select the folder of your images
  - Type the file ending into "File name contains:" (e.g .mrc, .tif)
  - Check "Use virtual stack"
  - Press OK Now you have read in your data as an image stack.

=== Reduce the size ===

For the sake of memory consumption and speed your should reduce the size / image depth of your image data.
  - Image -> Adjust -> Size
  - Set the width to 1024. The height is calculated automatically.
  - Press OK.
  - OPTIONAL - If your images shows very bad contrast (you cant see your particles) do the following:
    - Process -> Enhance Contrast
    - Set "Saturaed pixels" to 0.1%
    - Check "Process all X slices", uncheck everything else
    - Press OK
  - Image -> Type -> 8 Bit Wait until the processing is finished.

=== Save the file for later use ===
File -> Save As -> Tiff...

===== Parameters =====

==== Detection ====
=== Filament width ===
=== Mask width (Advanced option)=== 
=== Lower threshold ===
=== Upper threshold ===


==== Filtering ====

==== General ====

===== Step-by-Step Tutorial =====

User Tools

Site Tools

Page Tools