automated_helical_picking

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automated_helical_picking [2018/01/04 15:34]
twagner
automated_helical_picking [2018/06/20 13:12]
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-====== Helical Picking Procedure ====== 
- 
-===== Overview ===== 
-{{::​screen_shot_2017-12-08_at_12.43.57.png?​direct&​200 |}} The helical particle procedure ​ 
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-===== Installation ===== 
-===== Get your data into ImageJ ==== 
-=== Load the raw data === 
-  - File -> Import -> Image Sequence 
-  - Select the folder of your images 
-  - Type the file ending into "File name contains:"​ (e.g .mrc, .tif) 
-  - Check "Use virtual stack" 
-  - Press OK Now you have read in your data as an image stack. 
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-=== Reduce the size === 
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-For the sake of memory consumption and speed your should reduce the size / image depth of your image data. 
-  - Image -> Adjust -> Size 
-  - Set the width to 1024. The height is calculated automatically. 
-  - Press OK. 
-  - OPTIONAL - If your images shows very bad contrast (you cant see your particles) do the following: 
-    - Process -> Enhance Contrast 
-    - Set "​Saturaed pixels"​ to 0.1% 
-    - Check "​Process all X slices",​ uncheck everything else 
-    - Press OK 
-  - Image -> Type -> 8 Bit Wait until the processing is finished. 
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-=== Save the file for later use === 
-File -> Save As -> Tiff... 
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-===== Parameters ===== 
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-==== Detection ==== 
-=== Filament width === 
-=== Mask width (Advanced option)=== ​ 
-=== Lower threshold === 
-=== Upper threshold === 
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-==== Filtering ==== 
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-==== General ==== 
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-===== Step-by-Step Tutorial ===== 
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  • automated_helical_picking.txt
  • Last modified: 2018/06/20 13:12
  • (external edit)