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--- automated_helical_picking [2018/01/04 15:34]
twagner
+++ automated_helical_picking [2018/06/20 13:12]
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-====== Helical Picking Procedure ======
-===== Overview =====
-{{::screen_shot_2017-12-08_at_12.43.57.png?direct&200 |}} The helical particle procedure
-===== Installation =====
-===== Get your data into ImageJ ====
-=== Load the raw data ===
-  - File -> Import -> Image Sequence
-  - Select the folder of your images
-  - Type the file ending into "File name contains:" (e.g .mrc, .tif)
-  - Check "Use virtual stack"
-  - Press OK Now you have read in your data as an image stack.
-=== Reduce the size ===
-For the sake of memory consumption and speed your should reduce the size / image depth of your image data.
-  - Image -> Adjust -> Size
-  - Set the width to 1024. The height is calculated automatically.
-  - Press OK.
-  - OPTIONAL - If your images shows very bad contrast (you cant see your particles) do the following:
-    - Process -> Enhance Contrast
-    - Set "Saturaed pixels" to 0.1%
-    - Check "Process all X slices", uncheck everything else
-    - Press OK
-  - Image -> Type -> 8 Bit Wait until the processing is finished.
-=== Save the file for later use ===
-File -> Save As -> Tiff...
-===== Parameters =====
-==== Detection ====
-=== Filament width ===
-=== Mask width (Advanced option)===
-=== Lower threshold ===
-=== Upper threshold ===
-==== Filtering ====
-==== General ====
-===== Step-by-Step Tutorial =====