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automated_helical_picking [2017/12/11 17:45]
twagner
automated_helical_picking [2018/01/04 15:35]
twagner
Line 5: Line 5:
  
 ===== Installation ===== ===== Installation =====
 +===== Get your data into ImageJ ====
 +=== Load the raw data ===
 +  - File -> Import -> Image Sequence
 +  - Select the folder of your images
 +  - Type the file ending into "File name contains:" (e.g .mrc, .tif)
 +  - Check "Use virtual stack"
 +  - Press OK Now you have read in your data as an image stack.
 +
 +=== Reduce the size ===
 +
 +For the sake of memory consumption and speed your should reduce the size / image depth of your image data.
 +  - Image -> Adjust -> Size
 +  - Set the width to 1024. The height is calculated automatically.
 +  - Press OK.
 +  - Image -> Type -> 8 Bit Wait until the processing is finished.
 +
 +=== Save the file for later use ===
 +File -> Save As -> Tiff...
 +
 ===== Parameters ===== ===== Parameters =====
  
automated_helical_picking.txt ยท Last modified: 2018/06/20 13:12 (external edit)