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--- automated_helical_picking [2017/12/11 17:45]
twagner
+++ automated_helical_picking [2018/01/04 15:34]
twagner
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 ===== Installation =====
+===== Get your data into ImageJ ====
+=== Load the raw data ===
+  - File -> Import -> Image Sequence
+  - Select the folder of your images
+  - Type the file ending into "File name contains:" (e.g .mrc, .tif)
+  - Check "Use virtual stack"
+  - Press OK Now you have read in your data as an image stack.
+=== Reduce the size ===
+For the sake of memory consumption and speed your should reduce the size / image depth of your image data.
+  - Image -> Adjust -> Size
+  - Set the width to 1024. The height is calculated automatically.
+  - Press OK.
+  - OPTIONAL - If your images shows very bad contrast (you cant see your particles) do the following:
+    - Process -> Enhance Contrast
+    - Set "Saturaed pixels" to 0.1%
+    - Check "Process all X slices", uncheck everything else
+    - Press OK
+  - Image -> Type -> 8 Bit Wait until the processing is finished.
+=== Save the file for later use ===
+File -> Save As -> Tiff...
 ===== Parameters =====