Here we will put the sphire tutorial that is right now in available as pdf

General

1. Software Installation

1.1 Install sphire and MPI support

1.2 Install crYOLO

1.3 Installation of accociated EM Software Packages

2. Project

3. Movie

3.1 Micrograph Movie Alignement

3.2 Drift Asssessment

4. CTER

4.1 CTF Estimation

4.2 CTF Assessment

5. Window

5.1 Particle Coordinates

5.2 Particle Extraction

5.3 Particle Stack

6. ISAC

6.1 2D Clustering

6.2 Beautifier

6.3 ISAC2 Stack Subset

7. VIPER

7.1 Initial 3D Model - RVIPER

7.2 Compare re-projections

7.3 Volume Adjustment

7.4 Adaptive 3d Mask

8. MERIDIEN

8.1 3D Refinement

8.2 PostRefiner

9. SORT3D

9.1 3D Variability

9.2 3D Clustering

9.3 Local Subset Refinement and Final Reconstruction

10. Advanced Refinement

10.1 CTF Refinement

11. LOCALRES

11.1 Local Resolution

11.2 Local Filtering