ISAC2 Stack Subset: Create a virtual subset stack consisting of particles acounted for by ISAC2 by retrieving particle numbers associated with the ISAC2 or Beautifier class averages. The command also saves a selection file containing the retrieved original image numbers and 2D alignment parameters. In addition, it stores the 2D alignment parameters to the stack header.

Usage in command line

sp_pipe.py  isac_substack  input_bdb_stack_path  input_run_dir  output_directory  --isac_class_avgs_path=FILE_PATH  --substack_basename=SUBSTACK_BASENAME

sp_pipe isac_substack does not support MPI.

sp_pipe.py isac_substack 'bdb:outdir_window#data' 'outdir_pipe_sac_substack' --isac_class_avgs_path='ordered_class_averages_selected.hdf'

input_bdb_stack_path

Input bdb image stack: Specify the same bdb image stack used for the associated ISAC2 run. (default required string)

input_run_dir

ISAC2 or Beautifier run output directory: Specify output directory of an ISAC2 or Beautifier run as an input to this command. From this directory, the program extracts the shrink ratio and 2D alingment parameters of the ISAC2 run or local 2D alingment parameters of the Beautifier run. (default required string)

output_directory

Output directory: The results will be written here. It cannot be an existing one. (default required string)

--isac_class_avgs_path

ISAC2 or Beautifier class averages path: Specify path to a file containg ISAC2 or Beautifier class averages. The calss averages can be fullset or selected subset, as long as they are associated with the input bdb image stack and contain class member information stored in the headers. By default, the program uses the same deafult name of ordered class averages in ISAC2 or Beautifier (i.e. ordered_class_averages.hdf). (default none)

--substack_basename

Stack subset basename: Specify the basename of ISAC2 stack subset file. It cannot be empty string or only white spaces. (default isac_substack)

The following options are not implemented yet.

--isac_class_id

ISAC2 class average ID: Retrieve only particle members of the specifed ISAC2 class. By default, retrieve from all classes. (default -1)

--no_virtual_stack

Do not create virtual stack: Use this option to create only the particle ID list text file associated with the ISAC2 class averages. (default False)

--no_import_align2d

Do not import alignment: (default False)

Toshio Moriya

Category 1:: APPLICATIONS

sparx/bin/sp_pipe.py

sp_isac2

Beta:: Under evaluation and testing. Please let us know if there are any bugs.

There are no known bugs so far.