====== Helical Picking Procedure ====== ===== Overview ===== {{::screen_shot_2017-12-08_at_12.43.57.png?direct&200 |}} The helical particle procedure ===== Installation ===== ===== Get your data into ImageJ ==== === Load the raw data === - File -> Import -> Image Sequence - Select the folder of your images - Type the file ending into "File name contains:" (e.g .mrc, .tif) - Check "Use virtual stack" - Press OK Now you have read in your data as an image stack. === Reduce the size === For the sake of memory consumption and speed your should reduce the size / image depth of your image data. - Image -> Adjust -> Size - Set the width to 1024. The height is calculated automatically. - Press OK. - Image -> Type -> 8 Bit Wait until the processing is finished. === Save the file for later use === File -> Save As -> Tiff... ===== Parameters ===== ==== Detection ==== === Filament width === === Mask width (Advanced option)=== === Lower threshold === === Upper threshold === ==== Filtering ==== ==== General ==== ===== Step-by-Step Tutorial =====