Differences

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--- cryolo_picking_unlabeled [2019/03/16 11:58]
twagner [Toxin]
+++ cryolo_picking_unlabeled [2019/03/16 18:19]
twagner
@@ Line 53: / Line 53: @@
 Again, it still picks basically everything while avoiding contamination.
+===== TRPC4 =====
+The last example that I've choosen is TRPC4, as it contains much more contamination. The original training set comprises 32 images with 3038 particles (~94 particles / image):
+[{{:trpc_full.png?300|Example micrograph from the original training set.}}]
+Again, the same procedure as with toxin and ATP synthase. I removed 80% of particles randomly:
+[{{:trpc_selection.png?300|Example micrograph from the sparsely labeled training set.}}]
+I trained the model, and picked again. Here are the results for picking with the default threshold:
+[{{:trpc_03.png?300|Picking result with default threshold 0.3}}]
+It missed a lot, but picked far more that one would expect from the sparsely labeled training data. The missing particles appearing when you reduce the threshold to 0.14:
+[{{:trpc_014.png?300|Picking result with threshold 0.14}}]
+Particles picked, contamination skipped, mission accomplished :-)