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automated_helical_picking [2017/12/11 17:45] twagner |
automated_helical_picking [2018/01/04 15:35] twagner |
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===== Installation ===== | ===== Installation ===== | ||
+ | ===== Get your data into ImageJ ==== | ||
+ | === Load the raw data === | ||
+ | - File -> Import -> Image Sequence | ||
+ | - Select the folder of your images | ||
+ | - Type the file ending into "File name contains:" | ||
+ | - Check "Use virtual stack" | ||
+ | - Press OK Now you have read in your data as an image stack. | ||
+ | |||
+ | === Reduce the size === | ||
+ | |||
+ | For the sake of memory consumption and speed your should reduce the size / image depth of your image data. | ||
+ | - Image -> Adjust -> Size | ||
+ | - Set the width to 1024. The height is calculated automatically. | ||
+ | - Press OK. | ||
+ | - Image -> Type -> 8 Bit Wait until the processing is finished. | ||
+ | |||
+ | === Save the file for later use === | ||
+ | File -> Save As -> Tiff... | ||
+ | |||
===== Parameters ===== | ===== Parameters ===== | ||