automated_helical_picking
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automated_helical_picking [2017/12/08 11:48] twagner created |
automated_helical_picking [2018/01/04 15:34] twagner |
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| - | ====== | + | ====== Helical Picking Procedure ====== |
| ===== Overview ===== | ===== Overview ===== | ||
| + | {{:: | ||
| + | ===== Installation ===== | ||
| + | ===== Get your data into ImageJ ==== | ||
| + | === Load the raw data === | ||
| + | - File -> Import -> Image Sequence | ||
| + | - Select the folder of your images | ||
| + | - Type the file ending into "File name contains:" | ||
| + | - Check "Use virtual stack" | ||
| + | - Press OK Now you have read in your data as an image stack. | ||
| + | |||
| + | === Reduce the size === | ||
| + | |||
| + | For the sake of memory consumption and speed your should reduce the size / image depth of your image data. | ||
| + | - Image -> Adjust -> Size | ||
| + | - Set the width to 1024. The height is calculated automatically. | ||
| + | - Press OK. | ||
| + | - OPTIONAL - If your images shows very bad contrast (you cant see your particles) do the following: | ||
| + | - Process -> Enhance Contrast | ||
| + | - Set " | ||
| + | - Check " | ||
| + | - Press OK | ||
| + | - Image -> Type -> 8 Bit Wait until the processing is finished. | ||
| + | |||
| + | Save the file for later use | ||
| + | |||
| + | File -> Save As -> Tiff... | ||
| ===== Parameters ===== | ===== Parameters ===== | ||
| ==== Detection ==== | ==== Detection ==== | ||
| + | === Filament width === | ||
| + | === Mask width (Advanced option)=== | ||
| + | === Lower threshold === | ||
| + | === Upper threshold === | ||
| + | |||
| ==== Filtering ==== | ==== Filtering ==== | ||
| + | ==== General ==== | ||
| + | ===== Step-by-Step Tutorial ===== | ||
automated_helical_picking.txt ยท Last modified: 2018/06/20 13:12 (external edit)
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